Day One
Wednesday, December 11
8:00 am Registration & Morning Refreshments
8:50 am Chair’s Opening Remarks
Identifying Applications for Discovery & Engineering to Prioritize With Efficiency
9:00 am Fireside Chat: Weighing Up Benefits-Costs for Investment in Prediction Tools to Understand Potential vs Risk
Synopsis
• Evaluating prediction tool benefits to understand accelerated discovery effects
• Pin-pointing prediction tool limitations to understand overreliance risks and lack of flexibility issues
• Identifying how to mitigate associated prediction tool risks to decipher whether or not to invest
9:30 am Case Study: Optimizing Enzyme Engineering for a Cascade Reaction to Facilitate API Production
Synopsis
• Understanding how to develop enzymes accounting for substrate load and flexibility for a successful biocatalysis process
• Overcoming enzyme development challenges to obtain desired engineering outcomes
• Leveraging elements of ML to accelerate enzyme engineering
10:00 am Robust Biocatalyst Manufacturing Platform Backed by Nearly 50 Years of Experience – From Route Design to Ton-Scale Production
Synopsis
• Extensive Experience and Original Enzymes: Kaneka has extensive experience in industrial enzymatic reaction processes using a wide range of original enzymes
• Cost-Effective Processes: Kaneka has expertise with whole-cell biocatalysts reaction, which is one of the most cost-effective processes
• Case Study of Seamless Support: Kaneka has the capacity to provide route design, enzyme screening, enzyme engineering, scale-up study and ton-scale production seamlessly
Visualizing Upstream & Downstream Processes With a New Lens to Optimize End-to-End Process Efficiencies
10:30 am Tapping into Cascade Reactions for APIs to Minimize Waste & Support Green Chemistry
Synopsis
• Undertaking precision selection during engineering to promote enzyme compatibility with operating conditions
• Optimizing enzyme concentrations and reaction conditions to minimize the inhibition challenge
• Enabling coupled enzyme reactions to enable co-factor regeneration and streamline the cascade reaction
11:00 am Speed Networking & Morning Refreshments
Synopsis
Join our speed networking session to meet everyone in the room during a morning
refreshment period
11:45 am Futureproofing Early-Stage Scale up to Secure Optimal Synthesis Routes for Maximal End-Product Yields
Synopsis
• Making small-scale assessments to enable selectivity on a molecular scale
• Conducting small-scale synthesis planning to move forward with the most optimal biocatalysis routes
• Discovering routes for small and large scale and factoring in reproducibility and consistency
12:15 pm Optimizing Enzyme Activity, Selectivity, & Stability Through One-Shot Computational Design
Synopsis
• Scala offers one-shot enzyme design software
• Scala’s software optimizes enzyme activity, selectivity, and stability
• Scala’s technologies have been validated by over 50 research labs and top pharma companies
12:25 pm Case-Study: Uncovering Novo Nordisk’s Approach to Revolutionizing Oligonucleotide Production Via Biocatalysis & Observing Reactogenicity – Pharma Applications
Synopsis
• Implementing process efficiency for complex modalities and considering new developments for oligonucleotides to support biocatalysis optimization
• Synthesising shorter fragments during engineering to contribute towards sustainability and productivity of biocatalysis
• Hybridizing the oligonucleotide biocatalysis approach to maintain and maximize efficiency and yield
12:55 pm Networking Lunch
Narrowing Down Enzyme Variations Using Advanced Throughput Tools to Select for Flexibility
2:00 pm Identifying Non-Traditional Enzyme Engineering Capabilities to Improve Substrate Flexibility
Synopsis
• Exploring the technicalities of enzyme design for unconventional reactions to produce small molecules
• Pin-pointing optimal design principles during engineering to streamline early-stage success
• Understanding early conceptual framing to introduce non-conventional biocatalysis design
2:30 pm Enabling Biocatalysis Through Next-Generation DNA Synthesis
Synopsis
• Finding a good starting enzyme backbone has the potential to simplify and accelerate the workflow for biocatalysis optimization
• However, the cost of DNA synthesis can significantly limit the sequence space for enzyme backbones that can serve as a starting point in enzyme engineering
• Here, we present novel synthesis methods and screening products that have the potential to increase the breadth of “WT” starting points by up to 1000X
2:40 pm Roundtable Discussion: Identifying How to Streamline the Enzyme Discovery Process
Synopsis
- What is the largest barrier for enzyme discovery?
- What design principles should be prioritized in enzyme discovery vs. optimized later in enzyme engineering?
- Are we at a point with AI/ML technology to predict a subset of sequences with an enzyme class with a high probability of success for the target transformation?
3:15 pm Afternoon Break & Poster Session
Synopsis
Take part in this opportunity to present your recent work in the field, hear feedback and gain inspiration from industry pioneers in the room
Identifying Novel & Sophisticated Engineering Efforts With Industrial Promise for Small & Large Molecule Development
4:00 pm Case Study: Utilizing Computation in Engineering With Mutexa to Navigate the Large Design Landscape & Inform Engineering
Synopsis
• Leveraging automated workflows to allow computation-aided enzyme engineering to minimize time pressures and boost efficiency
• Exploring effective engineering efforts to allow enzymes to flourish at lower temperatures to decrease cost and environmental pressures
• Identifying emerging opportunities for engineering with computation to future-proof biocatalysis-informed drug development