Day Two

Thursday, November 13

8:00 am Coffee & Networking

8:50 am Chair’s Opening Remarks

Morning Workshop: Driving Faster Enzyme Engineering & Smarter Decisions with AI Innovations

Synopsis

This interactive workshop explores how AI and machine learning are transforming biocatalysis and enzyme engineering. Through a practical case study, expert presentations, and a roundtable discussion, attendees will gain insights into integrating AI tools, improving data practices, and navigating challenges in predictive modeling. The session emphasizes real-world applications, smarter experiment design, and setting priorities for the future of AI in biocatalysis.

9:00 am Unlocking AI/ML Potential Through End-to-End Data Integration

Synopsis

  • Identifying which data points and screening parameters provide the most impactful information for predictive modelling
  • Integrating activity and stability data in high-throughput screening to improve model accuracy
  • Designing experiments that facilitate smarter AI-driven enzyme optimization

9:30 am Smart, Scalable, and Scrappy: Building Affordable AI Workflows for Enzyme Engineering with Techno-Economic Impact in a Startup Environment

  • Leila Mashouf Co-Founder & Chief Technology Officer, Rubi Laboratories

Synopsis

  • Making AI-driven enzyme engineering more affordable for screening of larger libraries, generating richer datasets, and improving models for higher-quality outcomes
  • Building effective screening strategies within multi-enzyme cascades and using data to drive techno-economic decision making
  • Navigating the IP landscape and leveraging open-source tools to enable flexible and compliant AI-driven enzyme engineering

10:00 am Session Reserved for Scala Biodesign

10:10 am Roundtable Discussion: Making Sense of AI in Biocatalysis Today & Setting Priorities for Tomorrow

  • Jason Donald Senior Director - Protein Engineering, Stylus Medicine

Synopsis

  • Examining the current limitations and challenges in AI methods for enzyme and protein design
  • Exploring how companies are overcoming hurdles to make AI more practical and predictive
  • Assessing the real-world viability of AI technologies and identifying next steps for advancement

10:55 am Morning Break & Networking

Exploring Untapped Enzymes & Improving Engineering Success to Enhance Industrial Biocatalysis

11:30 am Exploring Untapped Enzyme Classes for New Industrially Relevant Reactions

  • Amelia Gilio Postdoctoral Research Fellow in Biocatalysis, Pfizer

Synopsis

  • Expanding substrate scope to unlock reactivity in underexplored enzyme classes or clades
  • Leveraging high-throughput screening and engineering to develop biocatalysts for industrial applications

12:00 pm Microheterogeneity on Enzyme: Formation, Analysis and Effects of Structural Variants

Synopsis

  • Understanding common mechanisms that lead to microheterogeneity, such as post-translational modifications (PTMs), degradation and modifications by reactive metabolites and excipients
  • Recognizing why microheterogeneity often goes undetected and learning analytical strategies to accurately assess protein quality
  • Discussing strategies to improve the success of enzyme engineering efforts by controlling microheterogeneity

12:30 pm Lunch Break

Combining Biocatalysis & Chemical Catalysis to Expand Synthetic Capabilities

1:30 pm Biocatalytic & Chemo-Enzymatic Strategies to Access Novel Amino Acids & Medicinally Relevant Small Molecules

Synopsis

  • Harnessing the unique selectivity of enzymes to solve longstanding challenges in chemical synthesis
  • Leveraging recent breakthroughs in enzyme engineering and genome mining to unlock new synthetic possibilities
  • Developing innovative hybrid strategies that integrate biocatalysis with modern chemical methodologies for the synthesis of noncanonical amino acids and medicinally relevant molecules

2:00 pm Bridging Biological and Chemical Approaches with Chemoenzymatic Catalysis

  • Sam Mallinson Researcher III, National Renewable Energy Laboratory (NREL)

Synopsis

  • Expanding the chemical space for synthetic co-factors and engineering enzymes to utilize these novel tools
  • Integrating chemical and enzymatic catalysis to develop advanced chemoenzymatic pathways
  • Bridging biocatalysis with applications in commodity chemicals and pharmaceuticals to achieve shared industrial goals

2:30 pm Afternoon Break & Networking

Advancing Biocatalytic Processes for Peptide Drugs Using Protein Engineering & Cascade Reactions

3:30 pm Protein Engineering Unlocks Biocatalytic Processes for a Peptide Drug

Synopsis

  • Details TBC

4:00 pm A Scalable Enzyme Cascade for the Synthesis of Non-Canonical Amino Acids in Peptide Drug Development

Synopsis

  • Developing a toolbox that allows for the rapid development of non-canonical amino acids
  • Leveraging cascade reactions to yield time and cost savings
  • Showcasing this reaction at the 50-100g scale

4:30 pm End of the 5th Applied Biocatalysis & Enzyme Engineering Summit